Background and Objective ?The inflammatory airway response in allergic asthma is very closely related to 12-lipoxygenases (12-LOXs). 12-LOXs produce a number of biologically active lipids, including 12-hydroxylated eicosatetraenoic acids (12(S)-HETE) involved in inflammation, and GPR31 (g-protein-coupled receptor 31) is thought to be a receptor for 12(S)-HETE.

Methods: The dynamic analysis of metabolomics and metabolic pathway-related indexes in dust mite allergic children was performed to determine the relationship between 12(S)-HETE and immune response in allergic asthma through the metabolic pathway intervention of allergen immunotherapy in a dust mite sensitized mouse model.

Results: It was found that 12(S)-HETE was significantly higher in house dust mite sensitized patients than in non-sensitized patients, and monitoring the inflammatory allergic response to immunotherapy in sensitized patients revealed that 12(S)-HETE was significantly lower after treatment. Hence, in our proposal, the 12-LOXs-12(S)-HETE-GPR31 signaling axis is a key mediator in maintaining immune homeostasis in asthmatic patients. 

Conclusion: 12(S)-HETE through 12-LOXs-12(s)-HETE-GPR31 signaling axis and allergic asthma immune response, and to elucidate the molecular regulatory mechanism of immunotherapy, providing a scientific basis for clinical diagnosis and treatment.