Objective:Allergies are the most common disease in children, and research has demonstrated that lipid metabolites,in combination with environmental exposure and genetic factors,can contribute to the sensitization process.This study investigated the metabolic profiles of serum from newborns and children with allergic diseases to understand the role of metabolites in the development of allergies.

Methods:From 2021 to 2022, all subjects were recruited at the First Affiliated Hospital of Guangzhou Medical University. Cord blood was obtained from the Department of Obstetrics. Serum was extracted from children with allergic diseases (including food allergies, asthma, allergic rhinitis,etc.)from pediatric respiratory medicine and healthy children.LC-MS was used to detect their serum, which included 362 metabolites of amino acid and fatty acid metabolites.

Results: 93 newborns and 34 healthy children,and 108 children with allergic diseases were recruited.A comparison of the metabolites in children with allergy and healthy.The Venn diagram revealed that children shared 83 different metabolites with allergic diseases.Upon KEGG enrichment,these metabolites were mainly associated with Aminoacyl-tRNA biosynthesis.Comparing the allergic disease and newborn groups,23 metabolites increased, with an enrichment in the Arachidonic acid metabolism pathway, and 56 decreased.The pathways most affected by the decreased metabolites were Aminoacyl-tRNA biosynthesis.

Conclusion:Apart from Arachidonic acid metabolism, Aminoacyl-tRNA biosynthesis is also a major contributor to the development of allergic diseases in newborns.It is worth exploring if regulating the synthesis of metabolites can be used as a means to prevent the occurrence of allergic diseases.