Title- Molecular characterization of drug? resistant strains of Mycobacterium tuberculosis

Background: Tuberculosis is a serious infectious disease affecting millions of people.  In fact, the increased cases of multi (MDR) and extreme drug resistance (XDR) worldwide remain the main challenge for tuberculosis control. This study presents the identification and characterization of resistant clinical isolates of Mycobacterium tuberculosis including a molecular resistance profile study by LPA.

Objectives:-

·         To examine the Prevalence of drug resistance Mycobacterium tuberculosis.

·         To evaluate drug resistance patterns in Mycobacterium tuberculosis isolates by FL-LPA and SL-LPA

 Method:- Sputum culture results from routine testing performed at the study sites are linked to the National TB Elimination Programme (NTEP) database, which was used to identify M. tuberculosis isolates from identified cases. Patient sputum smear examination and gene expert/ CBNAAT, LPA report will be taken by Nikshay portal.

Results: First line and Second line LPA was used to identify the variants causing resistance. Total cases tested by FL-LPA IS 1594. 986/1594(61.85%) isolates identified as m.tb detected, 27/1594(1.69%) m.tb +RIF, 93/1594(5.83%) m.tb +INH, 327/1594(20.51%) as MDR AND 161(10.10%) as other cases, A total of 348/717(48.53%) isolates were identified as Pre- XDR, 205/717(28.59%) as SLI resistant, 164/717(22.87%) others. However, an increase in the resistance to mono INH and fluoroquinolones was detected.

Conclusion: This work shows the highlighting need for the identification of emerging resistance for XDR detection. In this regard, we found an increase in fluoroquinolone resistance.