To analyse the metabolomic profile in high-risk subjects before LC diagnosis in the CHAIN and DEPRECAP cohort. M&M: Blood samples (BS) from subjects with LC at diagnosis (t0) were selected and compared with BS obtained in the years before LC diagnosis. These were classified according to the time of BS collection: three years before diagnosis (t-3) and from the 4th to the 7th year before LC diagnosis (t-4 to t-7). A metabolomic profile was analysed using advanced mass spectrometry and chromatography techniques. PLS-DA was used to compare the groups. RESULTS: 72 BS from 38 subjects were analysed. BS at t0 were compared with BS at the two periods before diagnosis [(t-3 and t-4 to 7)]. Significant differences were found in the analysis identifying 14 altered metabolites potentially implicated in developing LC, belonging to 8 metabolic pathways. When comparing samples at t0 with t-3, both groups were significantly different, identifying 14 potentially altered metabolites, mostly Oxalic acid; Valine; Stearic acid; Cholesterol; Phosphoric acid and Glutamine. When comparing blood samples at t0 and t-4 to t0 and t-4 to -7 beforehand, we also found significant differences (Figure 2) with the following metabolites: Glyceric acid; Acetamide; Glycine; Glutamine; Oxalic acid; Isocitric acid. The altered metabolites with the most significant impact on ROC curves in both analyses were oxalic acid, glutamine and glyceric acid. CONCLUSIONS: We found changing metabolomic profiles in the years before CP. Oxalic Acid, Glutamine and Glutamine and Glyceric Acid are the most relevant, regardless of the time before LC