Purpose:
   Reposition of Metformin as anti-tumor effect is well discussed among different cancer types including lung cancer. We attempt to explore anti-tumorigenesis pathway exerted by metformin in lung cancer management. We utilize the GSEA (Gene Set Enrichment Analysis ) as a useful tool for further approach.
 
Materials and Method:
  First, we designed lung cancer cell line (A549) exposure to metformin under normoxia or hypoxia status. Second, we harvested the cell line post treatment and extracted RNA and transfer to cDNA gradually. We analyzed the cDNA microarray by means of Gene Study Enrichment Analysis (GSEA) method. Among anti-angiogenesis and micro-environment engaged in lung cancer development and treatment were explored.
 
Results:
   Preliminary we find the metformin possess the anti-angiogenesis function by suppression hypoxia induced factor(HIF)-1? and PGK1 expression and proved by microarray (heat map) and western blot analysis. Further in vivo study is anticipated to elucidate the anti-tumor effect of metformin.  
 
Conclusion:
   We found Metformin can counteract the angiogenesis pathway, especially when exposure to hypoxia condition. Further approach the signal transduction and real mechanism between metformin and hypoxia environment involved in the anti-tumorigenesis needs further investigation.