ERS Respiratory Channel

CDN Connection Issue

It appears that your current network does not allow connection to the CDN (Content Delivery Network) required for this service to function properly.

To resolve this issue, please try the following actions :

• Contact your network administrator : Inform them of this restriction so they can adjust the network settings accordingly.

• Use another network : Try connecting from a different network, such as another Wi-Fi network or a mobile connection.

We apologize for the inconvenience and thank you for your understanding.

Abstract

Objectives: Nontuberculous mycobacterial lung disease (NTM-LD) and bronchiectasis have similar background lung structures. Both result in chronic persistent bacterial infection, but few studies have compared the microbiome composition of the two diseases.

Methods: In this exploratory study, we recruited patients with clinically suspected NTM-LD, performed bronchoscopy and collected bronchoalveolar lavage fluids (BALF). Patients were assigned to NTM-LD and bronchiectasis according to the bacteriological results of bronchoscopy.

Results: A total of 8 patients with NTM-LD and 11 patients with bronchiectasis were recruited. We compared the microbiome composition of BALF collected from them. In alpha diversity analysis, patients with NTM-LD presented a significantly higher Simpson index than those with bronchiectasis (H=5.734, P=0.0166). In beta diversity analysis, there were significant differences of Bray-Curtis distance (P=0.016) and Weighted UniFrac distance (P=0.002) between NTM-LD and bronchiectasis patients. A heat map analysis also showed the bacterial flora was clearly divided into two clusters by NTM-LD and bronchiectasis (Figure 1). It was also found that multiple characteristic strains of bacteria were significantly present in patients with NTM-LD. On the other hand, bacteria that were positive in culture tests in both NTM and bronchiectasis patients were not always detected in microbiome analysis.

Conclusions: There was a clear difference in microbiome composition between NTM-LD and bronchiectasis, which share common lung structures, with microbiome diversity maintained under infection with NTM.

Abstract

#{title}